Spatiotemporal Organization of Chemotaxis Pathways in Magnetospirillum gryphiswaldense
نویسندگان
چکیده
منابع مشابه
Transcriptional organization and regulation of magnetosome operons in Magnetospirillum gryphiswaldense.
Genes involved in magnetite biomineralization are clustered within the genomic magnetosome island of Magnetospirillum gryphiswaldense. Their transcriptional organization and regulation were studied by several approaches. Cotranscription of genes within the mamAB, mamDC, and mms clusters was demonstrated by reverse transcription-PCR (RT-PCR) of intergenic regions, indicating the presence of long...
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BACKGROUND AND OBJECTIVES The Magnetotactic bacterium Magnetospirillumgryphiswaldense (MSR-1) mineralizes the magnetite (Fe(3) O(4)) crystals and organizes a highly ordered intracellular structure, called the magnetosome. Iron transport system supports the biogenesis of magnetite. Although iron is an essential trace element for many metabolic pathways of the body, increase or decrease in iron w...
متن کاملIdentification of promoters for efficient gene expression in Magnetospirillum gryphiswaldense.
To develop an expression system for the magnetotactic bacterium Magnetospirillum gryphiswaldense, we compared gene expression from the widely used Escherichia coli P(lac) promoter with that from known and predicted genuine M. gryphiswaldense promoters. With the use of green fluorescent protein as a reporter, the highest expression level was observed with the magnetosomal P(mamDC) promoter. We d...
متن کاملComplete Genome Sequence of Magnetospirillum gryphiswaldense MSR-1
We report the complete genomic sequence of Magnetospirillum gryphiswaldense MSR-1 (DSM 6361), a type strain of the genus Magnetospirillum belonging to the Alphaproteobacteria. Compared to the reported draft sequence, extensive rearrangements and differences were found, indicating high genomic flexibility and "domestication" by accelerated evolution of the strain upon repeated passaging.
متن کاملSpatiotemporal organization, regulation, and functions of tractions during neutrophil chemotaxis.
Despite recent advances in our understanding of biochemical regulation of neutrophil chemotaxis, little is known about how mechanical factors control neutrophils' persistent polarity and rapid motility. Here, using a human neutrophil-like cell line and human primary neutrophils, we describe a dynamic spatiotemporal pattern of tractions during chemotaxis. Tractions are located at both the leadin...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2020
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.02229-20